The Impacts of Fermentation and Varied Leavening Agents on Phytic Acid Degradation and Mineral Nutritional Quality of Conventional Pakistani Flat Wheat Breads

Authors

  • Fazia Ghaffar Department of Food & Nutrition Sciences, College of Home Economics, University of Peshawar, Pakistan

DOI:

https://doi.org/10.70749/ijbr.v3i5.1269

Keywords:

Phytic Acid, Mineral Solubility, Phytate Degradation, Fermentation, Conventional Flat Breads

Abstract

Introduction: Wheat bread is a staple Pakistani food, and whole grain is generally thought of as being healthier than white bread due to its higher dietary fiber, vitamins, and many important minerals. However, wholegrain bread also contains high levels of phytate, which may bind desirable nutrients, preventing their absorption in the gut and thereby reducing the efficacy of the end product. Fermentation has been shown to decrease the amount of phytate in wholegrain cereals. The indigenous methods of leavening may be a potential biofriendly way of enhancing the nutritional quality of local wheat breads. Methods: The study aimed to evaluate the impact of heat processing on phytic acid, mineral content, and mineral solubility of conventional wheat breads. Six types of wheat flours were selected, including fine wheat flour, chakki wheat flour, maida, whole wheat flour, and granulated wheat flour. Initially, the raw flour was analyzed for percent phytate, phosphorus, and iron concentrations. Three types of breads: chapatti, flat bread made on an iron skillet, and tandoori roti (conventional hot oven), after preparing doughs as unleavened, fermented with bicarbonate soda, commercial yeast, and sourdough culture. All types of breads were analyzed for percent phytate, percent phytate degradation, mineral content, and mineral solubility as per standard procedures. Results: The results showed that raw fine wheat flour had the highest phytic content, while unleavened categories had the highest phytic acid content. The sourdough cultured leavened flour, the highest levels were observed as tawa Roti at 0.67% ± 3.30, chapatti at 0.99% ± 2.44, and tandoori Roti at 1.15% ± 2.29. The percentage degradation of phytate in fine wheat flour was highest in sourdough cultured tandoori roti at 61.8 %, followed by sourdough tawa roti at 61.0% and yeast tandoori roti at 60.47. The per cent degradation of phytate in Chakki wheat flour breads was highest in sourdough cultured tandoori roti (75.3), tawa Roti (70.1%), chapatti (57.5) and for the yeast fermented roti ithe higher degradation occurred in chapatti (55.4), tandoori roti (51.8) and tawa roti (48.2). soda tawa Roti (67.6 %), and yeast chapatti (0.3 %). Doughs leavened with soda also showed appreciable degradations. The percent degradation of phytate in maida wheat flour was highest in sourdough cultured tandoori roti (79.8%), tawa roti (69.5%), and chappati (58.5). The percent degradation of phytate in whole wheat flour was highest in sourdough cultured tandoori roti 57.7%, followed by tawa roti 47.6%, and chapatti 45.3%. Fermentation with the addition of bicarbonate soda also resulted in degradation. The unleavened breads showed the least reduction. The effects of fermentation were positive on both mineral content and mineral solubility. Conclusion: The current study concludes that the conventional method of making fermented wheat breads degrades phytate significantly while improving the mineral nutrition of these breads.

Downloads

Download data is not yet available.

References

Haudry A, Cenci A, Ravel C, Bataillon T, Brunel D, Poncet C, et al. Grinding up Wheat: a massive loss of nucleotide diversity since domestication. Mol Biol Evol. (2007) 24:1506–17. doi: 10.1093/molbev/msm077.

Wrigley C. Wheat: a unique grain for the world In: Khan K, Shewry PR, editors. Wheat: Chemistry and Technology. St. Paul, MN: American Association of Cereal Chemists; (2009). 1–17.

Gooding M, Khan K, Shewry P. The Wheat crop. Wheat: Chemistry and Technology. (2009). 4th Edn: Amsterdam: Elsevier, 19–49. [

Harlan JR. The Early History of Wheat. In: Evans LT, Peacock WJ, editors. Wheat Science Today and Tomorrow. Cambridge, UK: Cambridge University Press; 1981. pp. 1–9

Shuaib M, Zeb A, Ali Z, Ali W, Ahmad T, Khan I. Characterization of wheat varieties by seed storage protein electrophoresis. Afr J Biotechnol. 2007;6(5):497–500

Grote U, Fasse A, Nguyen TT, Erenstein O (2021) Food security and the dynamics of wheat and maize value chains in Africa and Asia. Frontiers in Sustainable Food Systems. https://doi.org/10.3389/fsufs.2020.617009

Chowdhry MA, Ramzan M, Alam K, Khaliq I. Correlation analysis for grain yield and quality traits in wheat. J Agric Res. 1995;33(2):71–74.

Igrejas G, Branlard G (2020) Wheat quality for improving processing and human health. Springer, Cham. https://doi.org/10.1007/978-3-030-34163-3

Bálint A, Szira F, Galiba G, Rakszegi M, Monostori I (2014) Micronutrient contents and nutritional values of commercial wheat flours and flours of field-grown wheat varieties—a survey in hungary. Cereal Res Commun Prepr Version. https://doi.org/10.1556/CRC.2013.0059

Central, U.S. Agricultural Research Service Food Data. (2021). Wheat germ nutritional value and analysis. https://nutrientoptimiser.com/nutritional-value-wheat-germ-crude/

Mahmoud A, Mohdaly A, Elneairy N (2015) Wheat germ: an overview on nutritional value antioxidant potential and antibacterial characteristics. J Food Nutr Sci 06:265–277. https://doi.org/10.4236/fns.2015.62027

Onipe OO, Jideani AIO, Beswa D (2015) Composition and Functionality of wheat bran and its application in some cereal food products. J Food Sci Technol 50:2509–2518. https://doi.org/10.1111/ijfs.12935

Hussain SS, Qamar R (2007) Wheat genomics: challenges and alternative strategies. Proc Pak Acad Sci 44:305–327

Brinch-Pedersen, H.; Madsen, C.K.; Holme, I.B.; Dionisio, G. Increased understanding of the cereal phytase complement for better mineral bio-availability and resource management. J. Cereal Sci. 2014, 59, 373–381.

Zimmermann, B.; Lantzsch, H.-J.; Langbein, U.; Drochner, W. Determination of phytase activity in cereal grains by direct incubation. J. Anim. Physiol. Anim. Nutr. 2002, 86, 347–352

Brouns, F. Phytic acid and whole grains for health controversy. Nutrients 2022, 14, 25.

Rodehutscord, M.; Sommerfeld, V.; Kühn, I.; Bedford, M.R. Phytases: Potential and limits of phytate destruction in the digestive tract of pigs and poultry. In Enzymes in Farm Animal Nutrition, 3rd ed.; Bedford, M.R., Partridge, G., Hruby, M., Walk, C.L., Eds.; CABI: Wallingford, UK, 2022; pp. 124–152. ISBN 9781789241563

Wang R., Guo S. Phytic acid and its interactions: Contributions to protein functionality, food processing, and safety. Compr Rev. Food Sci. Food Saf. 2021;20:2081–2105. doi: 10.1111/1541-4337.12714.

Raboy V. Low phytic acid Crops: Observations Based On Four Decades of Research. Plants. 2020;9:140. doi: 10.3390/plants9020140.

Raboy V. myo-Inositol-1,2,3,4,5,6-hexakisphosphate. Phytochemistry. 2003;64:1033–1043. doi: 10.1016/S0031-9422(03)00446-1

Brune M., Rossander-Hulten L., Hallberg L., Gleerup A., Sandberg A.S. Iron absorption from bread in humans: Inhibiting effects of cereal fiber, phytate and inositol phosphates with different numbers of phosphate groups. J. Nutr. 1992;122:442–449. doi: 10.1093/jn/122.3.442

Lopez H.W., Krespine V., Guy C., Messager A., Demigne C., Remesy C. Prolonged fermentation of whole wheat sourdough reduces phytate level and increases soluble magnesium. J. Agric. Food Chem. 2001;49:2657–2662. doi: 10.1021/jf001255z.

Buddrick O., Jones O.A., Cornell H.J., Small D.M. The influence of fermentation processes and cereal grains in wholegrain bread on reducing phytate content. J. Cereal Sci. 2014;59:3–8. doi: 10.1016/j.jcs.2013.11.006.

Yildirim R.M., Arici M. Effect of the fermentation temperature on the degradation of phytic acid in whole-wheat sourdough bread. LWT Food. Sci. Technol. 2019;112:108224. doi: 10.1016/j.lwt.2019.05.122

De Angelis M., Minervini F., Siragusa S., Rizzello C.G., Gobbetti M. Wholemeal wheat flours drive the microbiome and functional features of wheat sourdoughs. Int. J. Food Microbiol. 2019;302:35–46. doi: 10.1016/j.ijfoodmicro.2018.08.009.

Rodriguez-Ramiro I., Brearley C.A., Bruggraber S.F., Perfecto A., Shewry P., Fairweather-Tait S. Assessment of iron bioavailability from different bread making processes using an in vitro intestinal cell model. Food Chem. 2017;228:91–98. doi: 10.1016/j.foodchem.2017.01.130

Bottani M., Brasca M., Ferraretto A., Cardone G., Silvetti T. Chemical and nutritional properties of white bread leavened by lactic acid bacteria. J. Funct. Foods. 2018;45:330–338. doi: 10.1016/j.jff.2018.04.030

Zhao PH, Hou YC, Wang Z, Liao AM, Pan L, Zhang J, Dong YQ, Hu ZY, Huang JH, Ou XQ. Effect of fermentation on structural properties and antioxidant activity of wheat gluten by Bacillus subtilis. Front Nutr. 2023 Feb 23;10:1116982. doi: 10.3389/fnut.2023.1116982

Verni M, Rizzello CG, Coda R. Fermentation biotechnology applied to cereal industry by-products: nutritional and functional insights. Front Nutr. (2019) 6:42. doi: 10.3389/fnut.2019.00042

Tomassi, E., Arouna, N., Brasca, M., Silvetti, T., de Pascale, S., Troise, A. D., Scaloni, A., & Pucci, L. (2025). Fermentation of Whole-Wheat Using Different Combinations of Lactic Acid Bacteria and Yeast: Impact on In Vitro and Ex Vivo Antioxidant Activity. Foods, 14(3), 421. https://doi.org/10.3390/foods14030421

Sakandar, H.A.; Hussain, R.; Kubow, S.; Sadiq, F.A.; Huang, W.; Imran, M. Sourdough Bread: A Contemporary Cereal Fermented Product. J. Food Process. Preserv. 2019, 43, e13883.

Liu, J.; Yu, L.L.; Wu, Y. Bioactive Components and Health Beneficial Properties of Whole Wheat Foods. J. Agric. Food Chem. 2020, 68, 12904–12915.

Sharma, R.; Garg, P.; Kumar, P.; Bhatia, S.K.; Kulshrestha, S. Microbial Fermentation and Its Role in Quality Improvement of Fermented Foods. Fermentation 2020, 6, 106.

Garrido-Galand, S.; Asensio-Grau, A.; Calvo-Lerma, J.; Heredia, A.; Andrés, A. The Potential of Fermentation on Nutritional and Technological Improvement of Cereal and Legume Flours: A Review. Food Res. Int. 2021, 145, 110398

De Vuyst, L.; Neysens, P. The Sourdough Microflora: Biodiversity and Metabolic Interactions. Trends Food Sci. Technol. 2005, 16, 43–56

Kurtzman, C.P.; Robnett, C.J. Identification and Phylogeny of Ascomycetous Yeasts from Analysis of Nuclear Large Subunit (26S) Ribosomal DNA Partial Sequences. Antonie Van Leeuwenhoek 1998, 73, 331–371

Pérez-Alvarado, O.; Zepeda-Hernández, A.; Garcia-Amezquita, L.E.; Requena, T.; Vinderola, G.; García-Cayuela, T. Role of Lactic Acid Bacteria and Yeasts in Sourdough Fermentation during Breadmaking: Evaluation of Postbiotic-like Components and Health Benefits. Front. Microbiol. 2022, 13, 969460.

Mosharraf L., Effect of processing on the decrease in phytic acid and pesticide residue in wheat bran, Journal of Agricultural Engineering Research. (2015) 16, no. 2, 71–82.

Arya S. S. and Sonawane S. K., Impact of fiber mixture on dough and chapatti quality using D-optimal response surface methodology, Journal of Microbiology, Biotechnology and Food Sciences. (2016) 5, no. 5, 424–433, https://doi.org/10.15414/jmbfs.2016.5.5.424-433.

Nkhata SG, Ayua E, Kamau EH, Shingiro JB. Fermentation and germination improve nutritional value of cereals and legumes through activation of endogenous enzymes. Food Sci Nutr. 2018 Oct 16;6(8):2446-2458. doi: 10.1002/fsn3.846.

haven, J. K. , & Kadam, S. S. (1989). Nutritional improvement of cereals by fermentation. Critical Reviews in Food Science Technology, 28, 349–400. 10.1080/10408398909527507

Hassan, G. F. , Yusuf, L. , Adebolu, T. T. , & Onifade, A. K. (2015). Effect of fermentation on mineral and anti‐nutritional composition of cocoyam (Colocasia esculenta linn). Sky Journal of Food Science, 4, 042–049

Downloads

Published

2025-05-09

How to Cite

Ghaffar, F. (2025). The Impacts of Fermentation and Varied Leavening Agents on Phytic Acid Degradation and Mineral Nutritional Quality of Conventional Pakistani Flat Wheat Breads. Indus Journal of Bioscience Research, 3(5), 235–244. https://doi.org/10.70749/ijbr.v3i5.1269

Most read articles by the same author(s)