Molecular Characterization of Antibiotic Resistance Genes in Hospital-Acquired Urinary Tract Infections

Sumia Khan; Infal Malik; Laiba Khan; Fauzia Jan; Andrea Gangi; Ayesha Siddiqa; Taiba Tabassum; Muhammad Noman Khan; Nain Taara Bukhari

doi:10.70749/ijbr.v3i5.1459

Authors

Sumia Khan Department of Microbiology, Hazara University Mansehra, KP, Pakistan.
Infal Malik Pre-Medical, Steps College, Rawalpindi, Punjab, Pakistan.
Laiba Khan Center of Biotechnology and Microbiology, University of Peshawar, KP, Pakistan.
Fauzia Jan Department of Biochemistry, University Medical & Dental College Faisalabad, The University of Faisalabad, Punjab, Pakistan.
Andrea Gangi Department of Medicine, Faculty of Medicine and Health Sciences, Universitat Internacional de Catalunya, Barcelona, Spain.
Ayesha Siddiqa Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Punjab, Pakistan.
Taiba Tabassum Department of Physiology, Government College University Faisalabad, Punjab, Pakistan.
Muhammad Noman Khan Department of Microbiology, FATA University FR Kohat, Darra Adam Khel, KP, Pakistan.
Nain Taara Bukhari Department of Microbiology/Pathology, Bahria University of Health Sciences, Karachi Campus, Sindh, Pakistan.

DOI:

https://doi.org/10.70749/ijbr.v3i5.1459

Keywords:

Antibiotic Resistance Genes, Molecular Characterization, Hospital-Acquired Urinary Tract Infections, UTI, Hospital-Acquired UTI

Abstract

Introduction: Hospital-acquired urinary tract infections (HAUTIs) represent a major challenge in healthcare due to increasing multidrug resistance (MDR) among causative pathogens. Objective: This study aimed to molecularly characterize antibiotic resistance genes in bacterial isolates from HAUTI patients and assess their antimicrobial susceptibility profiles. Methodology: A total of 150 urine samples were collected from hospitalized patients diagnosed with HAUTIs. Bacterial isolates were identified using standard microbiological methods, and antibiotic susceptibility testing was performed following Clinical and Laboratory Standards Institute (CLSI) guidelines. Phenotypic screening for extended-spectrum beta-lactamase (ESBL) and carbapenemase production was conducted, followed by polymerase chain reaction (PCR) assays targeting key resistance genes including bla_CTX-M, bla_TEM, bla_SHV, bla_NDM, bla_OXA-48, and bla_KPC. Results: Out of 114 isolates recovered, Escherichia coli (40.4%) and Klebsiella pneumoniae (24.6%) were predominant. High resistance rates were observed against third-generation cephalosporins, fluoroquinolones, and carbapenems, with 68.4% of isolates classified as MDR. ESBL production was confirmed phenotypically in 57.0% of isolates, predominantly in E. coli and K. pneumoniae. Carbapenemase activity was detected in 25.4% of isolates, especially in K. pneumoniae and Acinetobacter baumannii. PCR analysis revealed bla_CTX-M as the most prevalent gene (66.7%), followed by bla_TEM (48.7%) and bla_NDM (26.9%). Conclusion: Co-occurrence of multiple resistance genes was common, highlighting the complexity of resistance mechanisms. The study underscores the critical need for continuous molecular surveillance and stringent antimicrobial stewardship to effectively manage HAUTIs and prevent the spread of resistant pathogens in healthcare settings.

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