Silver Nanoparticles as a Novel Therapeutic Approach for Bovine Mastitis: Efficacy and Comparative Analysis an Antimicrobial Perspective

Bilal Ahmed Khan; M Tariq Javed; Imtiaz Chemma; Sajid Ali Qaisrani; Muhammad Arif Bugti; Muhammad Bilal; Muhammad Mudassar; Obaid Muhammad Abdullah; Maryam Hanif; Nisar Ahmed Ujjan

doi:10.70749/ijbr.v3i5.1430

Authors

Bilal Ahmed Khan Department of Pathology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, Punjab, Pakistan/ Rural Poultry Development, Livestock and Dairy Development Department, Government of Balochistan, Spini Road, Quetta, Balochistan, Pakistan.
M Tariq Javed Perspective 1Department of Pathology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, Punjab, Pakistan.
Imtiaz Chemma Rural Poultry Development, Livestock and Dairy Development Department, Government of Balochistan, Spini Road, Quetta, Balochistan, Pakistan.
Sajid Ali Qaisrani Department of Veterinary Surgery, Faculty of Veterinary and Animal Sciences, Lasbela University of Agriculture, Water and Marine Sciences- Uthal, Balochistan, Pakistan
Muhammad Arif Bugti Institute of Microbiology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, Punjab, Pakistan.
Muhammad Bilal Disease Investigation Laboratory, Livestock and Dairy Development Department, Government of Balochistan, Spini Road, Quetta, Balochistan, Pakistan.
Muhammad Mudassar Department of Chemistry, Faculty of Sciences, Superior University Lahore, Punjab, Pakistan.
Obaid Muhammad Abdullah Department of Veterinary Surgery, University of Animal Sciences, Lahore, Punjab, Pakistan.
Maryam Hanif Department of Parasitology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, Punjab, Pakistan.
Nisar Ahmed Ujjan Faculty of Veterinary and Animal Sciences, Lasbela University of Agriculture, Water and Marine Sciences- Uthal, Balochistan, Pakistan.

DOI:

https://doi.org/10.70749/ijbr.v3i5.1430

Keywords:

Mastitis, Silver Nanoparticles, Antimicrobial Resistance, Dairy Cattle, Somatic Cell Count, Alternative Therapy

Abstract

Mastitis is one of the most prevalent and economically important diseases that affects dairy cattle globally. The disease leads to reduced milk production, poor animal health, and increased culling rates. Bacteria are the main cause of mastitis in dairy industry, where extensive antibiotic use has contributed to the emergence of antimicrobial resistance (AMR). To address this significant challenge, antimicrobial nanoparticles have emerged as promising alternative therapy. This study evaluates the efficacy of silver nanoparticles (AgNPs) in the treatment of bovine mastitis. A total of 40 lactating cattle diagnosed with mastitis were randomly divided into two groups (n=20). One of the groups received the treatment of intramammary administration of silver nanoparticles (100 µg) combined with gentamicin (60 mg) for five consecutive days, while the control group remained untreated. The Udder milk samples were collected before and after treatment to assess somatic cell count (SCC), bacterial load, pH, specific gravity, electrical conductivity, and malondialdehyde (MDA) concentration. The Results demonstrated a significant reduction in SCC and bacterial load in the nanoparticle-treated group compared to the controlled one. Additionally, improved milk quality parameters and reduced oxidative stress markers were observed. These findings indicate that silver nanoparticles exhibit potent antimicrobial properties and could serve as an effective therapeutic alternative for managing mastitis while mitigating the risk of AMR. Further studies are warranted to explore their long-term efficacy and safety in dairy herds.

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