Seroepidemiology and Molecular Detection of Brucellosis among Pakistani Women with Spontaneous Abortions

Afzal Ali; Hifsa Mobeen; Azeem Bibi; Insha Rafiq; Asma Fatima

doi:10.70749/ijbr.v3i5.1423

Authors

Afzal Ali Faculty of Allied Health Sciences, Superior University, Lahore, Pakistan.
Hifsa Mobeen Faculty of Allied Health Sciences, Superior University Lahore, Pakistan / PhD Scholar, Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Pakistan.
Azeem Bibi Faculty of Allied Health Sciences, Superior University, Lahore, Pakistan.
Insha Rafiq Faculty of Allied Health Sciences, Superior University, Lahore, Pakistan.
Asma Fatima Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Pakistan.

DOI:

https://doi.org/10.70749/ijbr.v3i5.1423

Keywords:

Brucellosis, Seroepidemiology, Molecular Detection, ELISA, RBPT, Abortion.

Abstract

Background: Brucellosis is a major public health hazard, especially in tropical and subtropical regions. The disease poses a considerable hazard because of its quick transmission and the possibility of severe sequelae. Objective: This study aims to determine the seroepidemiology and molecular detection of brucellosis among Pakistani women with spontaneous abortion. Methodology: A cross-sectional study was conducted in different hospitals of Lahore over four months. A total of 165 abortion cases were investigated for brucellosis using a sequential diagnostic approach involving the Rose Bengal Plate Test (RBPT), Enzyme-Linked Immunosorbent Assay (ELISA), and Polymerase Chain Reaction (PCR). Results: Initial screening with RBPT identified 41 (24.84%) positive cases, while 124 (75.15%) were negative. The RBPT positive samples were further evaluated by ELISA, which confirmed 34 (82.9%) as positive and 7 (17.0%) as negative. Subsequently, PCR analysis of the 34 ELISA-positive samples revealed 27 (79.4%) were positive for Brucella DNA, whereas 7 (20.5%) were PCR-negative. Conclusion: These findings indicate a confirmed brucellosis prevalence of 16.36% (27/165) among the abortion cases, underscoring the importance of combining serological and molecular diagnostics for accurate detection of Brucella infection.

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