Biological Investigation of Aqueous Extract of Funnel Seeds to Check its Therapeutic Effects in the Management of Polycystic Ovarian Syndrome in Induced PCOS Female Albino Rats

Maha Naseer; Ayesha Asad; Sajeela Akram; Kiran Aslam; Azka Momina Irfan; Fizza Niaz; Qura-tul-ain

doi:10.70749/ijbr.v2i02.253

Authors

Maha Naseer Department of Nutrition and Dietetics, The University of Faisalabad, Punjab, Pakistan.
Ayesha Asad Institute of Food Science and Technology, Sheffield Hallam University, Sheffield, England.
Sajeela Akram Department of Human Nutrition and Dietetics, University of Chakwal, Punjab, Pakistan.
Kiran Aslam Government Graduate College of Home Economics, Faisalabad, Punjab, Pakistan.
Azka Momina Irfan Department of Nutrition and Dietetics, The University of Faisalabad, Punjab, Pakistan.
Fizza Niaz Department of Nutrition and Dietetics, The University of Faisalabad, Punjab, Pakistan.
Qura-tul-ain Government Graduate College of Home Economics, Faisalabad, Punjab, Pakistan.

DOI:

https://doi.org/10.70749/ijbr.v2i02.253

Keywords:

Funnel Seeds, Ovarian Syndrome (PCOS), Follicle Stimulating Hormone, Luteinizing Hormone

Abstract

About every one out of nine women around the globe is living with polycystic ovarian syndrome. Polycystic ovarian syndrome is characterized by increased male hormone (androgen) in female body that results in delayed ovulation by unbalancing follicle stimulating hormone, luteinizing hormone, testosterone, and insulin levels. Other associated signs are increased insulin levels due to elevated androgens, weight gain, male pattern hair growth, and depression. Funnel seed is a wonder in regulating the imbalanced reproductive hormones. The purpose of Current study was to evaluate the hormone regulating properties by using funnel seed as source element. For this purpose, 14 female albino rats with induced polycystic ovarian syndrome were divided into two group. Treatment group was given funnel seed extract in dose of 50mg/kg of the body weight for sixty days. Levels of follicle stimulating hormone, luteinizing hormone, testosterone and insulin levels were measured and compared in blood samples on 0th day and 60th day in study groups. Insulin and testosterone levels were significantly (p<0.05) lower in the treatment group, whereas ovulating hormones, such as follicle stimulating hormone and luteinizing hormone, were significantly (p<0.05) higher.

References

Norman, R. J., Dewailly, D., Legro, R. S., & Hickey, T. E. (2007). Polycystic ovary syndrome. The Lancet, 370(9588), 685–697. https://doi.org/10.1016/s0140-6736(07)61345-2

Guzick, D. S. (2004). Polycystic Ovary Syndrome. Obstetrics & Gynecology, 103(1), 181–193. https://doi.org/10.1097/01.aog.0000104485.44999.c6

Ommen, S. R., Mital, S., Burke, M. A., Day, S. M., Deswal, A., Elliott, P., Evanovich, L. L., Hung, J., Joglar, J. A., Kantor, P., Kimmelstiel, C., Kittleson, M., Link, M. S., Maron, M. S., Martinez, M. W., Miyake, C. Y., Schaff, H. V., Semsarian, C., & Sorajja, P. (2020). 2020 AHA/ACC Guideline for the Diagnosis and Treatment of Patients With Hypertrophic Cardiomyopathy. Circulation, 142(25). https://doi.org/10.1161/cir.0000000000000937

Wolf, A. M., Fontham, E. T., Church, T. R., Flowers, C. R., Guerra, C. E., LaMonte, S. J., ... & Smith, R. A. (2018). Colorectal cancer screening for average‐risk adults: 2018 guideline update from the American Cancer Society. CA: a cancer journal for clinicians, 68(4), 250-281. https://doi.org/10.3322/caac.21457

Azziz, R. (2018). Polycystic ovary syndrome. Obstetrics & Gynecology, 132(2), 321-336. https://doi.org/10.1097/aog.0000000000002698

Khani, S., Abdollahi, M., Khalaj, A., Heidari, H., & Zohali, S. (2021). The effect of hydroalcoholic extract of Nigella Sativa seed on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: An experimental study. International Journal of Reproductive BioMedicine, 19(3), 271. https://doi.org/10.18502/ijrm.v19i3.8575

Ali, B. H., & Blunden, G. (2003). Pharmacological and toxicological properties of Nigella sativa. Phytotherapy Research: An international journal devoted to pharmacological and toxicological evaluation of natural product derivatives, 17(4), 299-305. https://doi.org/10.1002/ptr.1309

Kalleli, F., Bettaieb Rebey, I., Wannes, W. A., Boughalleb, F., Hammami, M., Saidani Tounsi, M., & M'hamdi, M. (2019). Chemical composition and antioxidant potential of essential oil and methanol extract from Tunisian and French fennel (Foeniculum vulgare Mill.) seeds. Journal of food biochemistry, 43(8), e12935. https://doi.org/10.1111/jfbc.12935

Ahmad, B. S., Talou, T., Saad, Z., Hijazi, A., Cerny, M., Kanaan, H., ... & Merah, O. (2018). Fennel oil and by-products seed characterization and their potential applications. Industrial crops and products, 111, 92-98. https://doi.org/10.1016/j.indcrop.2017.10.008

Hossain, M. M., Cao, M., Wang, Q., Kim, J. Y., Schellander, K., Tesfaye, D., & Tsang, B. K. (2013). Altered expression of miRNAs in a dihydrotestosterone-induced rat PCOS model. Journal of ovarian research, 6(1), 1-11. https://link.springer.com/article/10.1186/1757-2215-6-36

Damjanović, B., Lepojević, Ž., Živković, V., & Tolić, A. (2005). Extraction of fennel (Foeniculum vulgare Mill.) seeds with supercritical CO2: Comparison with hydrodistillation. Food Chemistry, 92(1), 143-149. https://doi.org/10.1016/j.foodchem.2004.07.019

Bhide, A., Shah, P. S., & Acharya, G. (2018). A simplified guide to randomized controlled trials. Acta obstetricia et gynecologica Scandinavica, 97(4), 380-387. https://doi.org/10.1111/aogs.13309

Ha, K. Y., Carragee, E., Cheng, I., Kwon, S. E., & Kim, Y. H. (2011). Pregabalin as a neuroprotector after spinal cord injury in rats: biochemical analysis and effect on glial cells. Journal of Korean medical science, 26(3), 404-411. https://doi.org/10.3346/jkms.2011.26.3.404

D’Agostino, R. B. (2017). Tests for the normal distribution. In Goodness-of-fit-techniques (pp. 367-420). Routledge. https://www.taylorfrancis.com/chapters/edit/10.1201/9780203753064-9/tests-normal-distribution-ralph-agostino

Moradi, S. Z., Momtaz, S., Bayrami, Z., Farzaei, M. H., & Abdollahi, M. (2020). Nanoformulations of herbal extracts in treatment of neurodegenerative disorders. Frontiers in bioengineering and biotechnology, 8, 238. https://doi.org/10.3389/fbioe.2020.00238

Khanage, S. G., Subhash, T. Y., & Bhaiyyasaheb, I. R. (2019). Herbal drugs for the treatment of polycystic ovary syndrome (PCOS) and its complications. Pharm. Res, 2(1), 5-13

. Poojar, B., Ommurugan, B., Adiga, S., & Thomas, H. (2017). Evaluation of antiurolithiatic property of ethanolic extract of fennel seeds in male wistar albino rats. Asian Journal of Pharmaceutical and Clinical Research, 10(8). http://dx.doi.org/10.22159/ajpcr.2017.v10i8.18923